Glomerular odorant mapping datasets

To understand how odors are represented in the initial stages of the mammalian olfactory system, we have developed a platform for efficiently mapping olfactory sensory neuron (OSN) input to glomeruli of the mouse olfactory bulb (OB). Using a high-throughput odorant delivery device (see ‘Olfactometry’) combined with the ultrasensitive genetically-encoded reporter GCaMP6s expressed in all OSNs, we have imaged OSN responses across glomeruli of the dorsal OB for 185 odorants, using odorant concentrations (1e-14 – 1e-9 M) chosen to identify the highest-sensitivity glomeruli for each odorant. The resulting datasets define consensus maps of odorant sensitivity across the dorsal OB, and also allow for the functional identification of numerous glomeruli based on extremely simple response criteria. An initial treatment of these data can be found in the poster presentation here, and the Symposium presentation at the BRAIN Initiative Investigators' 2021 meeting. We are currently (June 2021) preparing these data for publication and preprint sharing, but in the interim are making these datasets available on request. To submit a request, please fill out this form.

Data credits: Shawn Burton, Matt Wachowiak